Where should glass slides pipettes and capillary tubes be disposed?
Where should glass slides, pipettes, and capillary tubes be disposed? distilled water.
Where should you dispose of glass slides, syringes, used scalpel blades, and glass Pasteur pipettes? All laboratory chemicals can be disposed of by pouring them down the drain. Which of the following materials should be discarded in a biohazard bag?
Dispose of biohazardous waste in red bags. Only biohazardous waste should be placed inside red bags for disposal. Dispose of items such as plastic vagin*l speculums, used specimen swabs, used glucose test strips, urine dipsticks, blood-soaked drapes and gloves, and anything contaminated with OPIM in a red bag.
Autoclaves may be used to sterilize equipment/products prior to use in an experiment or to render items non-infectious prior to disposal. The University of Iowa's Biohazard Waste Guidelines states that cultures, plates, and vials containing pathogenic organisms must be autoclaved prior to disposal.
Every laboratory classroom should be equipped with a fire extinguisher, first aid kit, chemical shower, and eye wash station. Many also have a chemical fume hood and some may have a biosafety cabinet.
and graduated pipettes (5 mL, 10 mL, 25 mL, etc) may puncture through plastic trash bags, so these must also be disposed of in a specific manner. Non-contaminated pipette tips and graduated pipettes must be disposed of in a glassware box or a standard cardboard box lined with plastic to prevent leaks.
If slides, cover slips, and/or glass Pasteur pipets are contaminated with infectious agents or potentially infectious materials, dispose in sharps container (should be labeled “Biohazard”)
Used pipette tips should not be disposed of via the regular trash, as they can easily puncture the plastic trash bag and cause an injury. If the tip has been in contact with an infectious agent, it can be placed directly into a sharps container, or autoclaved and disposed of into the regulated medical waste box.
Today, the red biohazardous containers and bags seen throughout hospitals and doctors' offices are used to safely remove sharps, needles, and IV catheters that contain any human blood or bodily fluid.
Biological liquid waste can be poured down the drain (sanitary sewer), under running water after it has been decontaminated by autoclave or chemical means. Human or animal blood and body fluids do not need to be disinfected before being poured down the drain.
Which of the following should be disposed of in a biohazard bag quizlet?
What happens if a biohazard bag is full what should you do? You need to dispose of any culture in a glass test-tube, Any glass test tube used in the experiment, Any culture in a glass petri plate, and Any glass used Petri plate.
Sterile instruments and supplies should be stored in covered or closed cabinets. Dental instruments and supplies should not be stored under sinks or in other places where they might become wet.
All biological waste (noninfectious and infectious) generated during laboratory research must be deactivated by autoclaving or chemically treated before disposal. Sharps are items that are capable of puncturing, cutting or abrading the skin, e.g., needles, scalpel blades, slides and cover slips.
- Know locations of laboratory safety showers, eyewashstations, and fire extinguishers. ...
- Know emergency exit routes.
- Avoid skin and eye contact with all chemicals.
- Minimize all chemical exposures.
- No horseplay will be tolerated.
- Assume that all chemicals of unknown toxicity are highly toxic.
Handle and Store Glassware Safely
Never carry a beaker by its side. Always use two hands carrying any glassware (position one hand under the glass for support). Appropriate glove should be worn whe there is a risk of breakage (e.g. inserting a glass rod), chemical contamination, or thermal hazard.
The Most Important Lab Safety Rule
Know how to use all of the lab equipment before you begin. Why is this the most important rule? If you don't follow it: You endanger yourself and others in the lab.
The yellow container is for trace chemotherapy waste.
(You may also use a container marked as “trace chemotherapy.”) Trace contaminated chemotherapy waste is generated in the preparation & administration of antineoplastic/cytotoxic drugs.
Under most circ*mstances non-sharps waste that is potentially infectious, e.g. blood or other potentially infectious body fluids, may be disposed to the sewer without treatment 4. Disposal off-campus at a state-approved autoclave or incinerator.
Blades or any items used for cutting in a medical environment are considered Sharps (examples: scissors, knives, razors, scalpels) even if they have not been contaminated with biohazardous material. While glass and sharp plastic are considered sharps waste, their handling methods can vary.
Place only sharps (as defined in OSHA's Bloodborne Pathogens standard, for example, contaminated syringes, needles, lancets, scalpels, infusion needle sets, connection needles, auto-injectors) in sharps disposal containers.
Where would you discard a scalpel?
Needles, razor blades, scalpel blades, etc. can puncture cardboard boxes and must be disposed of in a plastic receptacle. If the sharps are also biohazardous, a red sharps container must be used. When full, these containers can be placed in the hazardous waste area of the lab and request a pick up by EHS (see below).
When discarding sharps: Never bend, shear, break, or recap disposable needles or remove from disposable syringes. Immediately following use, place the item into the sharps disposal container. Never reach into the sharps disposal container.
There are no laboratory glassware, plastics, or chemical reagent bottles that are recyclable at the time of this writing – do not place these items inside or near the recycling collection bins. It is always important to inform EH&S of any chemical contaminants in your waste streams.
Because of the liquids that scientists pipette, tips are NOT RECYCLABLE!
Non hazardous micropipette tips can be collected for recycling. Collect in a puncture proof container. BL1 and BL2 tips must be decontaminated with bleach or alcohol before being deposited into a recycling bin. Pharmaceutical contamination would also prevent recycling of tips.
The pollution board has also suggested three colours for dustbins — green for biodegradable waste; black for e-waste; and blue for plastic and metal waste.
In the US and other parts of the world, there are four major types of medical waste: General, Infectious, Hazardous and Radioactive.
Red biohazard bags are used to collect anatomical waste including human body fluids like sem*n, saliva, tissues, organs as well as animal carcasses. They are also used to dispose of the medical apparatus, specimen swabs, blood soaked drapes, gloves and anything that is visibly contaminated with blood or bodily fluids.
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- Any container that previously held a hazardous chemical or waste is defined as empty if:
- No hazardous materials can be poured, pumped or drained from the container, AND,
- No hazardous materials remain in the container that can be feasibly removed, AND,
Laboratory waste may disposed of in recycling, trash, laboratory glassware disposal boxes, sharps containers, or regulated medical waste boxes; it may need to be submitted to the Chemical Waste Program or Radioactive Waste Program pending contamination.
How do you dispose of blood samples in a lab?
Discard the denatured blood preferably via a sluice or laboratory sink with plenty of water. Any solids that are too large for the laboratory sink can be disposed of via biological waste.
Food Waste, Discarded Packaging and Electronics
Leftover food and discarded food packaging are not regulated biohazardous waste and should not be put in red bags. Food waste and packaging (and that includes drink cans and broken bottles) must be disposed of as municipal waste or recycled.
- Items contaminated with blood and other bodily fluids such as spinal, peritoneal and lymph fluids.
- Microbiological waste such as cultures, stocks and discarded biologicals, broths, inoculates, lab samples and live and attenuated vaccines.
Phlebotomists should dispose of these tubes in a sharps container after use. Gauze, bandages, and plastic tubes do not pose a skin-puncture risk.
Sterile supplies to be stored on open shelving (at least 250mm from the floor and 440mm below ceiling fixtures). Integrity of the sterile supply packaging is safeguarded by allowing space for stock of various shapes and sizes that will not compromise packaging and contents.
There's no need to remove the sterilized baby bottles and feed equipment from the steriliser or pan until you need to make a feed. Keeping the bottles in the steriliser or pan with the lid closed will keep them germ-free.
1.3 Store clean and sterile supplies in a designated area that is separate from other areas and is clean and dry, protected from dust, moisture (acceptable range 30-60% relative humidity) and temperature extremes (acceptable range 18-23°C).
fluids, human cell culture media, bacteria culture media, viruses, recombinant DNA, etc). Note: gloves, regardless of contaminated or not, and paper towels used for work surface or equipment decontamination must be disposed of in a clear autoclave bag or red biohazard bag.
Dispose of biohazardous waste in red bags. Only biohazardous waste should be placed inside red bags for disposal. Dispose of items such as plastic vagin*l speculums, used specimen swabs, used glucose test strips, urine dipsticks, blood-soaked drapes and gloves, and anything contaminated with OPIM in a red bag.
Biohazardous materials that are autoclaved should
be discarded as non-contaminated laboratory waste and placed in the regular trash or outside dumpster.
What are the do's and don'ts in laboratory?
Keep work area neat and free of any unnecessary objects. Thoroughly clean your laboratory work space at the end of the laboratory session. Do not block the sink drains with debris. Never block access to exits or emergency equipment.
- No Food or Drink.
- Wear Your PPE and Proper Lab Attire.
- Good Hygiene.
- Use Proper Storage Containers.
- Label Your Work Space.
- Don't Work Alone.
- Stay Focused and Aware of Your Surroundings.
- Participate in Safety Exercises.
- Round-bottom flasks should be stored with cork rings to keep them upright. ...
- Burettes and pipettes should be stored vertically in specially-designed racks, or horizontally in drawers.
- Place boxes on a steady, flat surface. Use good boxes. ...
- Don't place any heavy items on top of your fragile boxes.
- Avoid stacking your boxes too high. ...
- Keep your glassware and crockery at the back of your storage unit if you don't plan to use them any time soon.
Wash with hot soapy water, rinse thoroughly with tap water, then rinse 3-4 times with deionized water. Be sure the final rinses sheet off of the glass. Burets need to be thoroughly clean to be used for quantitative lab work. In some cases, you may need to soak the glassware overnight in soapy water.
Mostly acid-base mixing reactions are exothermic reactions which releases heat energy which can harm the person. So, it is advised to not mix acid and bases in the laboratory.
Solving the working alone dilemma
Rule #1: If at all possible, avoid working alone in a laboratory. If solo work is unavoidable, work during prime or peak hours or make sure someone is working nearby. Try enlisting a partner to work with you.
Many accidents can be prevented by wearing appropriate personal safety equipment. When working in a laboratory, you should wear a protective apron and gloves to protect your clothes and skin. You should also wear safety goggles or a face shield when performing any test where there is potential danger to the eyes.
Food Waste, Discarded Packaging and Electronics
Leftover food and discarded food packaging are not regulated biohazardous waste and should not be put in red bags. Food waste and packaging (and that includes drink cans and broken bottles) must be disposed of as municipal waste or recycled.
Red biohazard bags are used to collect anatomical waste including human body fluids like sem*n, saliva, tissues, organs as well as animal carcasses. They are also used to dispose of the medical apparatus, specimen swabs, blood soaked drapes, gloves and anything that is visibly contaminated with blood or bodily fluids.
What is a biohazard specimen bag used for?
Medical Specimen Bags
LPS Industries biohazard specimen bags are designed to protect healthcare workers from specimen leaks and accidental exposure to hazardous infections.
What goes in yellow biohazard bags? Yellow biohazard bags are used for the disposal of infectious waste, and materials that have been contaminated with infectious waste.
Yellow Bags – for infectious waste, including medicine contaminated infectious waste. This is waste that has been used in the treatments of infectious patients, those suspected of having an infection and are contaminated with medicines or chemicals. For example: PPE (gloves, masks aprons)
Red bag waste is biohazardous, or waste that is potentially infectious. It is also known as regulated medical waste and biohazardous waste.
Biohazardous waste, also called infectious waste (such as blood, body fluids, and human cell lines), is waste contaminated with potentially infectious agents or other materials that are deemed a threat to public health or the environment.
In the US and other parts of the world, there are four major types of medical waste: General, Infectious, Hazardous and Radioactive.
Yellow and black striped waste bags are also called tiger stripe bags. These are used for non-hazardous waste that is not infectious and doesn't contain harmful chemicals but is likely to be offensive to the senses. Examples: Colostomy bags, nappies, and sanitary waste.
Red biohazard bag. The color of a biohazardous bag is used to differentiate between waste that is regulated by DHS (red) and waste that is not (clear). The color of the bag does not indicate the level of biological risk or final treatment.
This type of packaging has secure, tamper-evident adhesive tape attached to them. They are used by many medical and healthcare facilities to hold and transport specimens for their laboratories. Other common names for them are lip-and-tape specimen bags, bio-hazard specimen bags, or lab specimen transport bags.